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Springer Protocols eBook Series

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  • Protein Post-Translational Modifications

    Methods and Protocols

    Edited by Xu Zhang, Rui Chen, Jianjun Li ...
    Series series Springer Protocols
    This detailed volume explores robust analytical workflows for post-translational modification (PTM) analysis in both basic and translational contexts. After brief foundational reviews of the field, the book delves into detailed experimental and computational protocols for traditional bottom-up strategies and top-down proteoform analysis, as well as PTM analysis of biotherapeutic proteins, ... Read more

    $179.09 USD

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  • Mass Spectrometry-Based Proteomics

    Edited by Kris Gevaert ...
    Series series Springer Protocols
    This detailed volume explores contemporary techniques in mass spectrometry-based proteomics. After covering overall proteome coverage and the cellular surfaceome, the book delves into proximity-induced biotinylation, abduction of protein complexes in viral-like particles, and thermal proteome profiling, as well as protocols for identifying protein N-terminal acetylation, protein processing by ... Read more

    $161.99 USD

  • Cryo-Electron Microscopy in Structural Biology

    From Structural Insights to Tomography and Drug Discovery

    Edited by Krishnarao Appasani ...
    Cryo-electron microscopy, in combination with tomography, has emerged as a new technology for visualizing molecular structures at a resolution beyond even 1 Å. Using this technology has revealed the native molecular details of viruses, membranes, enzymes, ribosomes, and cells. This comprehensive volume brings together authoritative overviews of these methods from structural and biological ... Read more

    $265.99 USD

  • Protein Termini Part B

    by David Christianson, Karen N. Allen, Thomas Arnesen ...
    Series Book 719 - Methods in Enzymology
    Protein termini represent a major route to protein regulation. From the moment the very first amino acid of a polypeptide chain exits the ribosome there is potential for steering from the cellular environment. This volume of Methods in Enzymology Modifications and Targeting of Protein Termini focuses on Protein N-termini and C-termini and their modifications which include acetylation, arginylation ... Read more

    $179.09 USD

  • Protein Termini Part A

    by Thomas Arnesen ...
    Series Book 718 - Methods in Enzymology
    Protein termini represent a major route to protein regulation. From the moment the very first amino acid of a polypeptide chain exits the ribosome there is potential for steering from the cellular environment. This volume of Methods in Enzymology Modifications and Targeting of Protein Termini focuses on Protein N-termini and C-termini and their modifications which include acetylation, arginylation ... Read more

    $179.99 USD

  • Activity-Based Proteomics

    Methods and Protocols

    Edited by Zhipeng Tao ...
    Series series Springer Protocols
    This volume comprehensively covers techniques that use activity-based proteomics to investigate biological functions of proteins such as lipolytic enzymes, serine hydrolases, chitinases, and glycoside hydrolases. The chapters in this book cover a wide range of protocols and an overview on the application of activity-based proteomics profiling to study deISGylating enzymes, serine hydrolases, ... Read more

    $224.99 USD

  • Post-translational Modifications That Modulate Enzyme Activity

    by Benjamin A. Garcia ...
    Series Book 626 - Methods in Enzymology
    Post-translational Modifications That Modulate Enzyme Activity, Volume 626 in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. Updated chapters include Crosstalk between cellular metabolism and histone acetylation, Isolation of protein complexes and modifications that regulate transcriptional machinery, High ... Read more

    $179.99 USD

  • Circular RNAs

    Edited by Christoph Dieterich, Marie-Laure Baudet ...
    Series series Springer Protocols
    This second edition details new and updated methods on circular RNA. Chapter guide readers through circular RNA purification, in silico characterization, circRNA detection, sequence validation, quantification , techniques related to gain- and loss-of-function approaches, circular RNA synthesis, split ligation, engineering, nanoparticle packaging, RNA modifications on circular RNA biogenesis, RNA ... Read more

    $197.99 USD

  • Selenoprotein Structure and Function

    by Eranthie Weerapana ...
    Series Book 662 - Methods in Enzymology
    Selenoprotein Structure and Function, Volume 662 in the Methods in Enzymology series, highlights new advances in the field, with this new volume presenting interesting chapters written by an international board of authors. Chapters in this new release include Identification of Selenoprotein O substrates using a biotinylated ATP analog, Selenium-encoded isotopic signature targeted profiling, ... Read more

    $179.99 USD

  • Structural Genomics and Drug Discovery

    Methods and Protocols

    Edited by Wayne F. Anderson ...
    Series series Springer Protocols
    Structural Genomics and Drug Discovery: Methods and Protocols focuses on high throughput structure determination methods and how they can be applied to lay the groundwork for structure aided drug discovery. The methods and protocols that are described can be applied in any laboratory interested in using detailed structural information to advance the initial stages of drug discovery. Written in the ... Read more

    $98.09 USD

  • Cell-Wide Identification of Metabolite-Protein Interactions

    Edited by Aleksandra Skirycz, Marcin Luzarowski, Jennifer C. Ewald ...
    Series series Springer Protocols
    This thorough volume explores protocols of proteome- and metabolome-wide strategies for the identification of protein-small molecule complexes in different organisms, in order to shed light on these important regulatory interactions. Experimental and computational strategies to characterize protein-metabolite interactions are discussed, and recent advances in enabling technologies are featured as ... Read more

    $98.99 USD

  • The Proteomics Protocols Handbook

    Edited by John M. Walker ...
    Series series Springer Protocols
    Hands-on researchers describe in step-by-step detail 73 proven laboratory methods and bioinformatics tools essential for analysis of the proteome. These cutting-edge techniques address such important tasks as sample preparation, 2D-PAGE, gel staining, mass spectrometry, and post-translational modification. There are also readily reproducible methods for protein expression profiling, identifying ... Read more

    $287.09 USD